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![GitHub release (latest by date)](https://img.shields.io/github/v/release/bcgsc/ntlink)
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![GitHub release (latest by date)](https://img.shields.io/github/v/release/BirolLab/ntlink)
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![Conda](https://img.shields.io/conda/dn/bioconda/ntlink?label=Conda)
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[![Published in BMC Bioinformatics](https://img.shields.io/badge/Published%20in-BMC%20Bioinformatics-blue)](https://doi.org/10.1186/s12859-021-04451-7)
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[![Published in Current Protocols](https://img.shields.io/badge/Published%20in-Current%20Protocols-blue)](https://doi.org/10.1002/cpz1.733)
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![Logo](https://github.com/bcgsc/ntLink/blob/master/ntlink-logo.png)
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![Logo](https://github.com/BirolLab/ntLink/blob/master/ntlink-logo.png)
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## Minimizer-based genome assembly scaffolding and mapping using long reads and minimizers
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The post-ntLink scaffolds file will have the suffix `*ntLink.scaffolds.fa`
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See our [wiki](https://github.com/bcgsc/ntLink/wiki) for more information about output file formats.
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See our [wiki](https://github.com/BirolLab/ntLink/wiki) for more information about output file formats.
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### Gap-filling
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To only run the pairing stage of `ntLink` (the stage where the long reads are mapped to the contigs), use the `pair` target for the `ntLink` command. The mappings can also be output in PAF-like format by specifying `paf=True`.
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**For more information about the ntLink algorithm and tips for running ntLink see our [wiki](https://github.com/bcgsc/ntLink/wiki)**
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**For more information about the ntLink algorithm and tips for running ntLink see our [wiki](https://github.com/BirolLab/ntLink/wiki)**
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## Installation
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ntLink is available from conda and homebrew package managers.
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Installing from source code:
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```
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curl -L --output ntLink-1.3.11.tar.gz https://github.com/bcgsc/ntLink/releases/download/v1.3.11/ntLink-1.3.11.tar.gz && tar xvzf ntLink-1.3.11.tar.gz
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curl -L --output ntLink-1.3.11.tar.gz https://github.com/BirolLab/ntLink/releases/download/v1.3.11/ntLink-1.3.11.tar.gz && tar xvzf ntLink-1.3.11.tar.gz
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```
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#### Testing your installation
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## Dependencies
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* Python 3.7+ ([Numpy](https://numpy.org/), [Python-igraph](https://igraph.org/python/))
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* [btllib](https://github.com/bcgsc/btllib) 1.6.2 or lower
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* [ABySS v2.3.0+](https://github.com/bcgsc/abyss)
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* [btllib](https://github.com/bcgsc/btllib) 1.7.1 or higher
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* [ABySS v2.3.0+](https://github.com/BirolLab/abyss)
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* GCC 6+ or Clang 5+ with OpenMP
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* [zlib](https://zlib.net/)
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